Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples
Human serum samples (n=28) were processed for Quinolinic Acid measurement by mean of both QA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9954).
Quinolinic acid ELISA kitRef: IS-I-0100
Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD+, a co-factor of many enzymatic reactions.
The Quinolinic Acid ELISA kit is optimized for the quantitation of Quinolinic Acid (QUIN or QA) within serum and plasma samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a low sample volume of 25µL, this assay enables pre-clinical and clinical sample testings.
< 6 ng/mL
|Range||25 - 2000 ng/mL|
|Assay time||Sample acylation 120 min, ELISA overnight|
|Species reactivity||Reacts with all species|
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|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
Quinolinic Acid ELISA kit
|Description||Competitive ELISA kit for the quantitative measurement of Quinolinic Acid in serum and plasma samples. For research use only.
|Minimal sample volume||25 µL
|Species reactivity||React with all species|
|Standard range||25 - 2000 ng/mL|
< 6 ng/mL
No significant cross-reactivity was observed with Quinolinic Acid analogs such as Kynurenic acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid
|Assay time||Sample acylation 120 min and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
|Sample collection & storage||
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample acylation (120 min)
|ELISA||Quinolinic acid antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on Quinolinic acid:
- Fertan et al. Effects of the Novel IDO Inhibitor DWG-1036 on the Behavior of Male and Female 3xTg-AD Mice. Front Pharmacol. 2019 Sep;24
- Pierozan et al. Synergistic Toxicity of the Neurometabolites Quinolinic Acid and Homocysteine in Cortical Neurons and Astrocytes: Implications in Alzheimer's Disease. Neurotox Res. 2018 Jul;34
- Hernandez-Martinez et al. Quinolinic acid induces neuritogenesis in SH-SY5Y neuroblastoma cells independently of NMDA receptor activation. Eur J Neurosci. 2017 Mar;45
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13