Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples
Human serum samples (n=28) were processed for Quinolinic Acid measurement by mean of both QA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9954).
Quinolinic acid ELISA kit
Ref: IS-I-0100
Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD+, a co-factor of many enzymatic reactions.
The Quinolinic Acid ELISA kit is optimized for the quantitation of Quinolinic Acid (QUIN or QA) within serum and plasma samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a low sample volume of 25µL, this assay enables pre-clinical and clinical sample testings.
| Sample type |
Serum, Plasma |
| Capacity | 96 tests |
| Sensitivity |
< 6 ng/mL |
| Range | 25 - 2000 ng/mL |
| Assay time | Sample acylation 120 min, ELISA overnight |
| Species reactivity | Reacts with all species |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Quinolinic Acid ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Quinolinic Acid in serum and plasma samples. For research use only. |
| Format | 96-well plate |
| Samples | Serum, Plasma |
| Minimal sample volume | 25 µL |
| Species reactivity | React with all species |
| Standard range | 25 - 2000 ng/mL |
| Sensitivity |
< 6 ng/mL |
| Specificity |
No significant cross-reactivity was observed with Quinolinic Acid analogs such as Kynurenic acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid |
| Assay time | Sample acylation 120 min and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage |
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Plasma (Heparin) Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation |
Sample acylation (120 min) |
| ELISA | Quinolinic acid antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Selected articles on Quinolinic acid:
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13
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