Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples Typical standard curve of Quinolinic acid ELISA Quinolinic Acid ELISA kit IS-I-0100 Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway

Cross-validation of Quinolinic Acid ELISA and LC/MS data in human serum samples

Human serum samples (n=28) were processed for Quinolinic Acid measurement by mean of both QA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9954).

Quinolinic acid ELISA kit

Ref: IS-I-0100

Quinolinic acid (QA) is a metabolite produced along the Kynurenine Pathway, which converts the aminoacid Tryptophan to NAD+, a co-factor of many enzymatic reactions.

The Quinolinic Acid ELISA kit is optimized for the quantitation of Quinolinic Acid (QUIN or QA) within serum and plasma samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a low sample volume of 25µL, this assay enables pre-clinical and clinical sample testings.

 

Sample type
Serum, Plasma
Capacity 96 tests
Sensitivity

< 6 ng/mL

Range 25 - 2000 ng/mL
Assay time Sample acylation 120 min, ELISA overnight
Species reactivity Reacts with all species

 

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Product information Key steps References Technical support Reviews

Product overview

Product name

Quinolinic Acid ELISA kit

Description Competitive ELISA kit for the quantitative measurement of Quinolinic Acid in serum and plasma samples. For research use only.
Format 96-well plate
Samples Serum
Minimal sample volume 25 µL
Species reactivity React with all species
Standard range 25 - 2000 ng/mL
Sensitivity

< 6 ng/mL

Specificity

No significant cross-reactivity was observed with Quinolinic Acid analogs such as Kynurenic acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid

Assay time Sample acylation 120 min and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet
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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample acylation (120 min)

ELISA Quinolinic acid antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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