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Immunostaining of 5-HT-containing cells in adult mouse brain slice

Anti-Serotonin antibody highlights the soma of serotoninergic neurons in the pons of mouse adult brain sagittal section. Staining was performed with STAINperfect immunostaining kit A, following the protocol for tissue sections. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.

Serotonin antibody (5-HT rabbit polyclonal Ab)

Ref: IS1007

 

The anti-Serotonin antibody IS1007 is a rabbit polyclonal antibody with high affinity and specificity. Using with the STAINperfect immunostaining kit A, our antibody labeled directly 5-HT in whole mounts, cell culture and tissue sections.

 

Clonality Polyclonal antibody
Host Rabbit
Species reactivity Reacts with all species
Tested samples whole mounts, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µL (approx. 40 tissue sections)
440
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Product overview


 

Product name

Serotonin antibody (5-HT rabbit polyclonal Ab)

Synonyms

Anti-5-HT antibody

Anti-5-hydroxytryptamine antibody

Immunogen Conjugated 5-HT
Specificity When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine.
Volume 50µL
Lot number

140301

 

Storage


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for Serotonin staining
Protocols-at-a-glance

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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