Immunostaining of 5-HT-containing cells in adult mouse brain slice
Anti-Serotonin antibody highlights the soma of serotoninergic neurons in the pons of mouse adult brain sagittal section. Staining was performed with STAINperfect immunostaining kit A, following the protocol for tissue sections. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
Serotonin antibody (5-HT rabbit polyclonal Ab)
Ref: IS1007
The anti-Serotonin antibody IS1007 is a rabbit polyclonal antibody with high affinity and specificity. Using with the STAINperfect immunostaining kit A, our antibody labeled directly 5-HT in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Species reactivity | Reacts with all species |
| Tested samples | whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
Serotonin antibody (5-HT rabbit polyclonal Ab) |
| Synonyms |
Anti-5-HT antibody Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine. |
| Volume | 50µL |
| Lot number |
140301 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Serotonin staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
References
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers about serotonin:
- Gershon MD. 5-Hydroxytryptamine (serotonin) in the gastrointestinal tract. Curr Opin Endocrinol Diabetes Obes. 2013 Feb;20(1):14-21. doi: 10.1097/MED.0b013e32835bc703.
- Baganz NL, Blakely RD. A dialogue between the immune system and brain, spoken in the language of serotonin. ACS Chem Neurosci. 2013 Jan 16;4(1):48-63. doi: 10.1021/cn300186b. Epub 2012 Dec 7.
- Bortolato M et al. The role of the serotonergic system at the interface of aggression and suicide. Neuroscience. 2013 Apr 16;236:160-85.
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