Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain
Immunodetection of serotonin (red – IS040) and Tyrosine hydroxylase (green) in the mouse embryonic midbrain (E13.5) using mouse monoclonal anti-serotonin antibody (IS040) and rabbit polyclonal anti-TH antibody, following protocol for whole mount samples provided with STAINPerfect kit A (SP-A-1000).
Serotonin antibody (5-HT mouse monoclonal Ab)Ref: IS040
Anti-Serotonin antibody IS040 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels 5-HT in whole mounts, cell culture and tissue sections.
|Applications||IHC / IF
|Species reactivity||Reacts with all species|
|Tested samples||whole mounts, cell culture, tissue sections
|Staining procedure||STAINperfect immunostaining kit A|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
Serotonin antibody - mouse mAb
|Specificity||When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine.
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
|Material safety datasheet||Download MSDS
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A.Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for Serotonin staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers about serotonin:
- Gershon MD. 5-Hydroxytryptamine (serotonin) in the gastrointestinal tract. Curr Opin Endocrinol Diabetes Obes. 2013 Feb;20(1):14-21. doi: 10.1097/MED.0b013e32835bc703.
- Baganz NL, Blakely RD. A dialogue between the immune system and brain, spoken in the language of serotonin. ACS Chem Neurosci. 2013 Jan 16;4(1):48-63. doi: 10.1021/cn300186b. Epub 2012 Dec 7.
- Bortolato M et al. The role of the serotonergic system at the interface of aggression and suicide. Neuroscience. 2013 Apr 16;236:160-85.
- Kiyasova V et al. A genetically defined morphologically and functionally unique subset of 5-HT neurons in the mouse raphe nuclei. J Neurosci. 2011 Feb 23;31(8):2756-68. doi: 10.1523/JNEUROSCI.4080-10.2011.