Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain Serotonin neurons and fibers in embryonic mouse medulla Affinity & specificity of anti-Serotonin antibody Serotonin - 5-hydroxytryptamine (5-HT)

Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain

Immunodetection of serotonin (red – IS040) and Tyrosine hydroxylase (green) in the mouse embryonic midbrain (E13.5) using mouse monoclonal anti-serotonin antibody (IS040) and rabbit polyclonal anti-TH antibody, following protocol for whole mount samples provided with STAINPerfect kit A (SP-A-1000).

Serotonin antibody (5-HT mouse monoclonal Ab)

Ref: IS040

 

Anti-Serotonin antibody IS040 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels 5-HT in whole mounts, cell culture and tissue sections.

 

Clonality Monoclonal antibody
Host Mouse
Applications IHCIF
Species reactivity Reacts with all species
Tested samples whole mounts, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µL

440
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Product information Protocols References Technical support Reviews

Product overview


 

Product name

Serotonin antibody - mouse mAb

Synonyms

Anti-5-HT antibody

Anti-5-hydroxytryptamine antibody

Immunogen Conjugated 5-HT
Specificity When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine.
Volume 50µL
Lot number

 

 

 

Storage


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles

Material safety datasheet Download MSDS

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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A.Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for Serotonin staining
Protocols-at-a-glance

 

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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