Antibodies & immunoassays for small molecules L-Tryptophan ELISA kit
Any sample
Ref: BA-E-2700
Tryptophan metabolismAmino acidsDiagnostic ELISA kits (IVD)ELISA
+Tryptophan ELISA: Standard curve
Standard curve - to be generated every time the assay is performed.
DatasheetMSDSProtocol
This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.
| Sample type | Biological fluids (urine, plasma, serum, …) and cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | < 1.2µg/ml |
| Range | 2.5 – 250µg/ml |
| Assay time | 2h30 sample preparation and ELISA overnight |
| Reactivity | Reacts with all species |
| References | Citations in the litterature |
Product overview
| Product name | Tryptophan ELISA kit |
| Description | In vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | urine, plasma, serum, cell culture supernatant |
| Sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 2.5 – 250µg/ml |
| Sensitivity |
< 1.2µg/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine |
| Assay time | Sample preparation 2.5 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use , Material safety datasheet |
| Kits intended for in-vitro diagnostic (IVD) are not available for the US-market.
In contrast to kits for in-vitro-diagnostic (IVD), kits meant for research use only (RUO) do have a R in the last position of the catalog number. |
| Sample collection & storage | Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)
Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples |
| Sample preparation | Sample precipitation (15 min) and derivatization (2h15) |
| ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
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Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
2022-08 - Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
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Authors : Kim et al., Oncoimmunology
2021-11 - Apathy is not associated with a panel of biomarkers in older adults with HIV disease
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Authors : Wood et al., Journal of psychosomatic research
2021-11 - Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
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Authors : Pospíšková et al., Pteridines
2021-11 - Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
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Authors : Jung Cho et al., European Respiratory Journal
2021-05 - Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan
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Authors : Cho et al., journal of Immunology
2021-03 - Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner
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Authors : Park et al., International journal of molecular sciences
2021-02 - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
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Authors : Masachika et al., Clinical neuroscience
2020-06 - Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
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Authors : Watanabe et al., Clinical Cancer Research
2019-11 - Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
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Authors : Walker et al., Molecular Psychiatry
2018-07 - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
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Authors : Martin-Hernandez et al., Molecular Neurobiology
2018-05 - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
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Authors : Toulmonde et al., JAMA oncology
2018-01 - In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
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Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
2018-01 - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
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Authors : Ziklo et al., Frontiers in cellular and infection microbiology
2018-01 - In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp
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Authors : Ziklo et al. BMC Microbiology
2016-12
