L-Tryptophan ELISA kit
Any sample

Ref: BA-E-2700
Tryptophan metabolismAmino acidsDiagnostic ELISA kits (IVD)ELISA
DatasheetMSDSProtocol

This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.

Sample type Biological fluids (urine, plasma, serum, …) and cell culture supernatant
Capacity 96 tests
Sensitivity < 1.2µg/ml
Range 2.5 – 250µg/ml
Assay time 2h30 sample preparation and ELISA overnight
Reactivity Reacts with all species
References Citations in the litterature

 

Size

1 x 96 wells

410.00

100 in stock

Quantity
Total

410.00

Worldwide shipping

Select delivery location

Product overview

Product name Tryptophan ELISA kit
Description In vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants
Labels IVD, CE
Format 96-well plate
Samples urine, plasma, serum, cell culture supernatant
Sample volume 20µL
Reactivity Reacts with all species
Standard range 2.5 – 250µg/ml
Sensitivity

< 1.2µg/ml

Specificity No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine
Assay time Sample preparation 2.5 hours and ELISA overnight
Storage Store at 2-8°C for to 6 months
Datasheets Instructions for use Material safety datasheet

 

Kits intended for in-vitro diagnostic (IVD) are not available for the US-market.

In contrast to kits for in-vitro-diagnostic (IVD), kits meant for research use only (RUO) do have a R in the last position of the catalogue number.
The research kit can be ordered with the catalogue no. BA E-2700R.

Sample collection & storage Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)

Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples

Sample preparation Sample precipitation (15 min) and derivatization (2h15)
ELISA Tryptophan antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citation

  • IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
    Check the article
    Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
    2022-08
  • Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
    Check the article
    Authors : Kim et al., Oncoimmunology
    2021-11
  • Apathy is not associated with a panel of biomarkers in older adults with HIV disease
    Check the article
    Authors : Wood et al., Journal of psychosomatic research
    2021-11
  • Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
    Check the article
    Authors : Pospíšková et al., Pteridines
    2021-11
  • Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
    Check the article
    Authors : Jung Cho et al., European Respiratory Journal
    2021-05
  • Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan
    Check the article
    Authors : Cho et al., journal of Immunology
    2021-03
  • Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner
    Check the article
    Authors : Park et al., International journal of molecular sciences
    2021-02
  • Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
    Check the article
    Authors : Masachika et al., Clinical neuroscience
    2020-06
  • Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
    Check the article
    Authors : Watanabe et al., Clinical Cancer Research
    2019-11
  • Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
    Check the article
    Authors : Walker et al., Molecular Psychiatry
    2018-07
  • Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
    Check the article
    Authors : Martin-Hernandez et al., Molecular Neurobiology
    2018-05
  • Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
    Check the article
    Authors : Toulmonde et al., JAMA oncology
    2018-01
  • In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
    Check the article
    Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
    2018-01
  • Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
    Check the article
    Authors : Ziklo et al., Frontiers in cellular and infection microbiology
    2018-01
  • In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp
    Check the article
    Authors : Ziklo et al. BMC Microbiology
    2016-12

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