Plasma/Urine catecholamines ELISA kit: Dopamine, Adrenaline, Noradrenaline Plasma/Urine catecholamines ELISA kit: Dopamine, Adrenaline, Noradrenaline Fast 3 catecholamines ELISA: Standard curves

Plasma/Urine catecholamines ELISA kit: Dopamine, Adrenaline, Noradrenaline

3 Catecholamines ELISA kit - Urine & Plasma - Fast

Ref: BA-E-6600

 

This Catecholamine ELISA kit is tailored for the rapid measurement of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine) and Dopamine in urine and plasma samples. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables to determine catecholamine levels in up to 40 samples within 4 hours.

 

Sample type
10 µl urine, 300 µl plasma
Samples/kit 40 samples in duplicate
Sensitivity

Epinephrine: 0.3 ng/ml urine, 10 pg/ml plasma Norepinephrine: 1.5 ng/ml urine, 40 pg/ml plasma Dopamine: 4.5 ng/ml urine, 25 pg/ml plasma

Range

Epinephrine: 0 / 1 - 200 ng/ml
Norepinephrine: 0 / 5 - 1 000 ng/ml
Dopamine: 0 / 4.5 - 2 000 ng/ml

Assay time Sample extraction and acylation (65 min), ELISA (3h)
Species reactivity Reacts with all species
Citations 2
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Product information Key steps References Technical support Reviews

Product overview

Product name

3 catecholamines ELISA kit - Urine/Plasma - Fast-Track

Description In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples.
Labels IVD, CE
Format 3x96-well plate
Samples Urine, Plasma
Sample volume 10 µl urine, 300 µl plasma
Cross specie-reactivity React with all species
Standard range Epinephrine: 0 / 1 - 200 ng/ml 
Norepinephrine: 0 / 5 - 1 000 ng/ml 
Dopamine: 0 / 4.5 - 2 000 ng/ml
Sensitivity

Epinephrine: 0.3 ng/ml urine, 10 pg/ml plasma Norepinephrine: 1.5 ng/ml urine, 40 pg/ml plasma Dopamine: 4.5 ng/ml urine, 25 pg/ml plasma

Specificity No significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay time 4 hours
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample preparation, extraction and acylation (65min)

ELISA Dopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h).
Detailed protocol Download instructions for use
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