Plasma/Urine Histamine ELISA Plasma/Urine Histamine ELISA Plasma/Urine Histamine ELISA: Standard curve

Plasma/Urine Histamine ELISA

Histamine ELISA kit - Urine-Plasma - Fast Track

Ref: BA-E-1000

 

This Histamine ELISA kit is tailored for the quantitative measurement of histamine in urine and plasma samples. Intended for in vitro diagnostics, the immunoassay can be combined with the add-on kit BA-E-1100, to quantify histamine release in whole blood.

 

Sample type
10 µl urine, 25 µl plasma
Samples/kit 40 samples in duplicate
Sensitivity

0.12 ng/ml plasma, 0.3 ng/ml urine

Range 0 / 0.5 - 50 ng/ml
Assay time

Sample acylation (1h) and ELISA overnight

Species reactivity Reacts with all species
Citations 4
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Product information Key steps References Technical support Reviews

Product overview

Product name

Histamine ELISA kit - Urine and Plasma samples

Description In vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in urine and plasma samples.
Labels IVD, CE
Format 96-well plate
Samples 10 µl urine, 25 µl plasma
Cross specie-reactivity React with all species
Standard range 0 / 0.5 - 50 ng/ml
Sensitivity

0.12 ng/ml plasma, 0.3 ng/ml urine

Specificity

No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin

Assay time 1h sample acetylation + ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for use Material safety datasheet
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Key steps

Sample collection & storage

Plasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample acylation (1h)

ELISA Histamine antiserum incubation (3H or overnight), revelation and read steps (1h).
Detailed protocol Download instructions for use
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