Urine Metanephrines ELISA Urine Metanephrines ELISA Urinary metanephrines ELISA: Standard curves

Urine Metanephrines ELISA

Metanephrine-Normetanephrine ELISA kit - Urine - Fast Track

Ref: BA-E-8600

 

This Metanephrines ELISA kit enables to quantify metanephrine and normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics, this quantitative immunoassay demonstrated good correlation with HPLC.

 

Sample type
25 µl urine
Samples/kit 40 samples in duplicate
Sensitivity

13 ng/ml - Metanephrine
23 ng/ml - Normetanephrine

Range 0 / 20 - 2 000 ng/ml - Metanephrine
0 / 30 - 3 000 ng/ml - Normetanephrine

Assay time Sample hydrolysis and acetylation (45 min), ELISA (1h)
Species reactivity Reacts with all species
Citations 2
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Product information Key steps References Technical support Reviews

Product overview

Product name

Metanephrine-Normetanephrine ELISA kit - Urine- Fast-Track

Description In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples.
Labels IVD, CE
Format 96-well plate
Samples Urine
Sample volume 25 µL
Cross specie-reactivity React with all species
Standard range 0 / 20 - 2 000 ng/ml - Metanephrine
0 / 30 - 3 000 ng/ml - Normetanephrine
Sensitivity

13 ng/ml - Metanephrine
23 ng/ml - Normetanephrine

Specificity No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine.
Assay time Sample hydrolysis and acetylation 45 min and ELISA (1)
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months)

Sample preparation

Sample hydrolysis (30 min) and acetylation (15min)

ELISA Metanephrine antiserum incubation (30 min), revelation and read steps (30h).
Detailed protocol Download instructions for use
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