Urine Metanephrines ELISA
Metanephrine-Normetanephrine ELISA kit - Urine - Fast Track
Ref: BA-E-8600
This Metanephrines ELISA kit enables to quantify metanephrine and normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics, this quantitative immunoassay demonstrated good correlation with HPLC.
| Sample type |
25 µl urine |
| Samples/kit | 40 samples in duplicate |
| Sensitivity |
13 ng/ml - Metanephrine |
| Range | 0 / 20 - 2 000 ng/ml - Metanephrine 0 / 30 - 3 000 ng/ml - Normetanephrine |
| Assay time | Sample hydrolysis and acetylation (45 min), ELISA (1h) |
| Species reactivity | Reacts with all species |
| Citations | 2 |
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Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Metanephrine-Normetanephrine ELISA kit - Urine- Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | Urine |
| Sample volume | 25 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 20 - 2 000 ng/ml - Metanephrine 0 / 30 - 3 000 ng/ml - Normetanephrine |
| Sensitivity |
13 ng/ml - Metanephrine |
| Specificity | No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine. |
| Assay time | Sample hydrolysis and acetylation 45 min and ELISA (1) |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage |
Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
| Sample preparation |
Sample hydrolysis (30 min) and acetylation (15min) |
| ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h). |
| Detailed protocol | Download instructions for use |
References
- Unger N. et al. Plasma and Urinary Metanephrines Determined by an Enzyme Immunoassay, but not Serum Chromogranin A for the Diagnosis of Pheochromocytoma in Patients with Adrenal Mass. Exp Clin Endocrinol Diabetes. 2012 Sep;120(8):494-500.
- Marrone O. Blood pressure changes after automatic and fixed CPAP in obstructive sleep apnea: relationship with nocturnal sympathetic activity. Clin Exp Hypertens. 2011;33(6):373-80.
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