Kynurenic acid detection in human brain by IF Kynurenic acid detection in human brain by IF Kynurenic acid detection in human brain by IHC Affinity & specificity of anti- Kynurenic acid antibody Kynurenic acid

Kynurenic acid detection in human brain by IF

Immunohistofluorescence reveals kynurenic acid accumulation in the cytoplasm of human glial cells in human caudate-putamen. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary anti-Kynurenic acid antibody (dilution 1/250). After incubation with Alexa-555 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize the staining.

Kynurenic acid antibody

Ref: IS010

 

The monoclonal 4G12-A12 anti-Kynurenic acid antibody was validated for IHC and IF in human caudate putamen tissues. Competitive ELISA demonstrated the antibody to be highly affine and specific.

 

Clonality Monoclonal antibody (clone 4G12-A12)
Host Mouse
Validated applications IHC / IF
Specie reactivity Reacts with all species
References Not yet cited to our knowledge. 
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Product information Protocols References Technical support Reviews

Product overview

 

Product name Kynurenic acid antibody
Synonyms

Kinurenic acid antibody

4-Hydroxyquinoline-2-carboxylic acid antibody

KYNA antibody

Immunogen Conjugated kynurenic acid
Isotype IgG1 k chain
Clone clone 4G12-A12
Specificity When tested in competitive ELISA, the anti-Kynurenic antibody 4G12-A12 did not show any significant cross reactivity with Quinaldic, Xanthurenic, Anthranilic, Picolinic or Quinolinic acid conjugates
Lot number

140201

 

 

Storage

 

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
Material safety datasheet

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Protocols

Immunohistochemistry (IHC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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References

Antibody not yet cited. Submit an article and get a 10% discount.

 

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