Kynurenic acid detection in human caudate putamen by IHC
Immunohistochemistry (IHC) reveals kynurenic acid accumulation in the cytoplasm of glial cells in human caudate putamen. Paraffin-embedded brain tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with the primary anti-kynurenic acid polyclonal antibody (dilution 1/2000). After incubation with polymer-conjugated secondary Ab, DAB was used to visualize the staining.
Kynurenic acid polyclonal antibodyRef: IS1011
The anti-Kynurenic acid (KYNA) rabbit polyclonal antibody proved to work at 1/2000 dilution on paraffin-embedded tissues, a single vial thus catering for approximately 400 stainings.
|Host||Rabbit (see anti-KYNA mouse mAb)|
|Specie reactivity||Reacts with all species|
|References||Not yet cited to our knowledge.
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|Product information||Protocols||References||Technical support||Reviews|
|Product name||Kynurenic acid polyclonal antibody|
Kinurenic acid polyclonal antibody4-Hydroxyquinoline-2-carboxylic acid polyclonal antibody
KYNA polyclonal antibody
|Immunogen||Conjugated kynurenic acid
|Specificity||When tested in competitive ELISA, the anti-Kynurenic polyclonal antibody did not show any significant cross reactivity with Quinaldic and Xanthurenic conjugates.
||Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
|Material safety datasheet
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only|
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Selected articles on Kynurenic acid:
- Justinova Z et al. Reducing cannabinoid abuse and preventing relapse by enhancing endogenous brain levels of kynurenic acid. Nat Neurosci. 2013 Nov;16(11):1652-61. doi: 10.1038/nn.3540. Epub 2013 Oct 13.
- Stone TW, Stoy N, Darlington LG. An expanding range of targets for kynurenine metabolites of tryptophan. Trends Pharmacol Sci. 2013 Feb;34(2):136-43. doi: 10.1016/j.tips.2012.09.006. Epub 2012 Nov 1.
- Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.
- Moroni F, Cozzi A, Sili M, Mannaioni G. Kynurenic acid: a metabolite with multiple actions and multiple targets in brain and periphery. J Neural Transm. 2012 Feb;119(2):133-9. doi: 10.1007/s00702-011-0763-x. Epub 2012 Jan 4.
- Linderholm KR et al. Increased levels of kynurenine and kynurenic acid in the CSF of patients with schizophrenia. Schizophr Bull. 2012 May;38(3):426-32. doi: 10.1093/schbul/sbq086. Epub 2010 Aug 20.
- Stone TW, Forrest CM, Darlington LG. Kynurenine pathway inhibition as a therapeutic strategy for neuroprotection. FEBS J. 2012 Apr;279(8):1386-97. doi: 10.1111/j.1742-4658.2012.08487.x. Epub 2012 Mar 27.