Quinaldic acid detection by IF in human brain
Quinaldic acid visualization by immunofluorescence in human caudate putamen. Staining illustrates cytoplasmic accumulation of Quinaldic acid in glial cells. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/5O0). After incubation with Alexa-555 conjugated secondary Ab, epifluorescence microscopy (10X) was used to capture the staining.
Quinaldic acid antibody
Ref: IS004
Our anti-Quinaldic acid antibody is a mouse monoclonal antibody, which was validated for immunohistochemistry (IHC) and immufluorescence (IF) in paraffin-embedded tissues from human caudate-putamen region.
| Clonality | Monoclonal antibody (clone 5A4-H10) |
| Host | Mouse |
| Valided applications | IHC / IF |
| Specie reactivity | Reacts with all species |
| References | Not yet cited to our knowledge Submit content and get a 10% discount! |
| Format | 50µL |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name | Quinaldic acid antibody |
| Synonyms |
2-Quinolinecarboxylic acid antibody Quinolin-2-carboxylate acid antibody |
| Immunogen | Conjugated Quinaldic acid |
| Isotype | IgG1 k chain |
| Clone | clone 5A4-H10 |
| Specificity | When tested in competitive ELISA, the anti-Quinaldic acid antibody did not show any significant cross reactivity with Kynurenic and Xanthurenic acid conjugates |
| Lot number |
140201 |
Storage
| Form |
Liquid |
| Purity | Purified IgG |
| Concentration |
0,5 mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| Immunohistochemistry (IHC) |
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
References
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publication on Quinaldic acid:
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