Quinaldic acid detection by IF in human brain Quinaldic acid detection by IF in human brain Quinaldic acid detection by IHC in human brain Affinity & Specificity of anti-Quinaldic acid antibody Quinaldic acid

Quinaldic acid detection by IF in human brain

Quinaldic acid visualization by immunofluorescence in human caudate putamen. Staining illustrates cytoplasmic accumulation of Quinaldic acid in glial cells. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/5O0). After incubation with Alexa-555 conjugated secondary Ab, epifluorescence microscopy (10X) was used to capture the staining.

Quinaldic acid antibody

Ref: IS004

 

Our anti-Quinaldic acid antibody is a mouse monoclonal antibody, which was validated for immunohistochemistry (IHC) and immufluorescence (IF) in paraffin-embedded tissues from human caudate-putamen region.

 

Clonality Monoclonal antibody (clone 5A4-H10)
Host Mouse
Valided applications IHC / IF
Specie reactivity Reacts with all species
References Not yet cited to our knowledge
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Product overview

 

Product name Quinaldic acid antibody
Synonyms

2-Quinolinecarboxylic acid antibody

Quinolin-2-carboxylate acid antibody

Immunogen Conjugated Quinaldic acid
Isotype IgG1 k chain
Clone clone 5A4-H10
Specificity When tested in competitive ELISA, the anti-Quinaldic acid antibody did not show any significant cross reactivity with Kynurenic and Xanthurenic acid conjugates
Lot number

140201

 

 

Storage

 

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

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Protocols

Immunohistochemistry (IHC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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