Quinolinic acid detection in human midbrain by immunohistofluorescence (IHF) Quinolinic acid detection in human midbrain by immunohistofluorescence (IHF) Quinolinic acid detection in human midbrain by immunohistochemistry (IHC) Anti- Quinolinic acid antibody affinity & specificity Quinolinic acid

Quinolinic acid detection in human midbrain by immunohistofluorescence (IHF)

Immunofluorescence staining highlights nuclear exclusion of Quinolinic acid in human midbrain. Paraffin-embedded brain tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary anti-Quinolinic acid antibody (dilution 1/250). After incubation with Alexa-555 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize the staining.

Quinolinic acid antibody

Ref: IS002

 

Confirmed to be highly specific and affine by competitive ELISA, the monoclonal anti- Quinolinic acid antibody 4E11-G3 was validated for immunofluorescence (IF) and immunohistochemistry (IHC) in human midbrain tissues.

 

Clonality Monoclonal antibody (clone 4E11-G3)
Host Mouse
Valided applications IHC / IF
Specie reactivity Reacts with all species
References Not yet cited to our knowledge
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Product information Protocols References Technical support Reviews

Product overview

 

Product name Quinolinic acid antibody
Synonyms

Pyridine-2,3-dicarboxylic acid antibody

2,3-pyridinedicarboxylic acid antibody

3,4-Pyridinedicarboxylic acid antibody

Pyridine-3,4-dicarboxylic acid antibody
Immunogen Conjugated quinolinic acid
Isotype IgG1 k chain
Clone clone 4E11-G3
Specificity When tested in competitive ELISA, the anti-Quinolinic acid antibody did not show any significant cross reactivity with Picolinic and Quinaldic acid conjugates
Lot number

140201

 

Storage

 

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

Immunohistochemistry (IHC)

Dilute at 1:100-1:1000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate fluorescent dyes conjugated secondary antibody

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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