Quinolinic acid detection in human midbrain by immunohistofluorescence (IHF)
Immunofluorescence staining highlights nuclear exclusion of Quinolinic acid in human midbrain. Paraffin-embedded brain tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary anti-Quinolinic acid antibody (dilution 1/250). After incubation with Alexa-555 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize the staining.
Quinolinic acid antibody
Ref: IS002
Confirmed to be highly specific and affine by competitive ELISA, the monoclonal anti- Quinolinic acid antibody 4E11-G3 was validated for immunofluorescence (IF) and immunohistochemistry (IHC) in human midbrain tissues.
| Clonality | Monoclonal antibody (clone 4E11-G3) |
| Host | Mouse |
| Valided applications | IHC / IF |
| Specie reactivity | Reacts with all species |
| References | Not yet cited to our knowledge Submit content and get a 10% discount! |
| Format | 50µL |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name | Quinolinic acid antibody |
| Synonyms |
Pyridine-2,3-dicarboxylic acid antibody 2,3-pyridinedicarboxylic acid antibody 3,4-Pyridinedicarboxylic acid antibody Pyridine-3,4-dicarboxylic acid antibody |
| Immunogen | Conjugated quinolinic acid |
| Isotype | IgG1 k chain |
| Clone | clone 4E11-G3 |
| Specificity | When tested in competitive ELISA, the anti-Quinolinic acid antibody did not show any significant cross reactivity with Picolinic and Quinaldic acid conjugates |
| Lot number |
140201 |
Storage
| Form |
Liquid |
| Purity | Purified IgG |
| Concentration |
0,5 mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| Immunohistochemistry (IHC) |
Dilute at 1:100-1:1000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate fluorescent dyes conjugated secondary antibody |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
References
Antibody not yet cited. Submit an article and get a 10% discount!
Selected publications about Quinolinic acid:
- Lugo-Huitrón R, Ugalde Muñiz P, Pineda B, Pedraza-Chaverrí J, Ríos C, Pérez-de la Cruz V. Quinolinic acid: an endogenous neurotoxin with multiple targets. Oxid Med Cell Longev. 2013;2013:104024. doi: 10.1155/2013/104024. Epub 2013 Sep 5. Review.
- Wu W, Nicolazzo JA, Wen L, Chung R, Stankovic R, Bao SS, Lim CK, Brew BJ, Cullen KM, Guillemin GJ. Expression of tryptophan 2,3-dioxygenase and production of kynurenine pathway metabolites in triple transgenic mice and human Alzheimer's disease brain. PLoS One. 2013 Apr 22;8(4):e59749. doi: 10.1371/journal.pone.0059749. Print 2013
- Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.
- Guillemin GJ.Quinolinic acid, the inescapable neurotoxin.FEBS J. 2012 Apr;279(8):1356-65. doi: 10.1111/j.1742-4658.2012.08485.x. Epub 2012 Mar 27. Review
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