Quinolinic acid detection in human parkinson's brain
Immunohistochemistry (IHC) reveals the presence of Quinolinic acid in glial cells in human parkinsonian midbrain tissue. Paraffin-embedded tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary anti-quinolinic acid polyclonal antibody (dilution 1/1000). After incubation with polymer-conjugated secondary Ab, DAB was used to visualize the staining.
Quinolinic acid polyclonal antibodyRef: IS1010
Validated for IHC in human brain tissues, the anti-Quinolinic acid (QUIN) rabbit polyclonal antibody proved to work at 1/1000 dilution on paraffin-embedded sections, a single vial thus catering for approximately 200 stainings.
|Specie reactivity||Reacts with all species|
|References||Not yet cited to our knowledge
Submit content and get a 10% discount!
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
|Product name||Quinolinic acid polyclonal antibody|
Pyridine-2,3-dicarboxylic acid polyclonal antibody
Anti-quinolinate polyclonal antibody
Anti-QUIN polyclonal antibody
|Immunogen||Conjugated quinolinic acid
|Specificity||When tested in competitive ELISA, the anti-Quinolinic acid polyclonal antibody did not show any significant cross reactivity with Picolinic and anthranilic acids conjugates|
|Storage||Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
|Material safety datasheet||Download MSDS|
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only|
Selected publications about Quinolinic acid:
- Lugo-Huitrón R et al. Quinolinic acid: an endogenous neurotoxin with multiple targets. Oxid Med Cell Longev. 2013;2013:104024. doi: 10.1155/2013/104024. Epub 2013 Sep 5. Review.
- Wu W et al. Expression of tryptophan 2,3-dioxygenase and production of kynurenine pathway metabolites in triple transgenic mice and human Alzheimer's disease brain. PLoS One. 2013 Apr 22;8(4):e59749. doi: 10.1371/journal.pone.0059749. Print 2013
- Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.
- Guillemin GJ.Quinolinic acid, the inescapable neurotoxin.FEBS J. 2012 Apr;279(8):1356-65. doi: 10.1111/j.1742-4658.2012.08485.x. Epub 2012 Mar 27. Review