Quinolinic acid detection in human parkinson's brain Quinolinic acid detection in human parkinson's brain Affinity & specificity of anti-Quinolinic polyclonal antibody Quinolinic acid (QUIN)

Quinolinic acid detection in human parkinson's brain

Immunohistochemistry (IHC) reveals the presence of Quinolinic acid in glial cells in human parkinsonian midbrain tissue. Paraffin-embedded tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary anti-quinolinic acid polyclonal antibody (dilution 1/1000). After incubation with polymer-conjugated secondary Ab, DAB was used to visualize the staining.

Quinolinic acid polyclonal antibody

Ref: IS1010

 

Validated for IHC in human brain tissues, the anti-Quinolinic acid (QUIN) rabbit polyclonal antibody proved to work at 1/1000 dilution on paraffin-embedded sections, a single vial thus catering for approximately 200 stainings.

 

Clonality Polyclonal antibody
Host Rabbit
Valided applications IHC
Specie reactivity Reacts with all species
References Not yet cited to our knowledge
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Product information Protocols References Technical support Reviews

Product overview

 

Product name Quinolinic acid polyclonal antibody
Synonyms

Pyridine-2,3-dicarboxylic acid polyclonal antibody

Anti-quinolinate polyclonal antibody

Anti-QUIN polyclonal antibody

Immunogen Conjugated quinolinic acid
Specificity When tested in competitive ELISA, the anti-Quinolinic acid polyclonal antibody did not show any significant cross reactivity with Picolinic and anthranilic acids conjugates
Lot number

140401



Storage

 

Form

Liquid

Purity Purified anti-serum
Storage Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
Material safety datasheet Download MSDS
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Protocols

Immunohistochemistry (IHC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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