Antibodies & immunoassays for small molecules Dopamine rabbit pAb – IS1005
Ref: IS1005-sp
NeuromediatorsCatecholaminesPolyclonal AntibodiesICCIFIHC
+Dopamine imaging in mouse embryonic mesencephalic neurons (E13.5)
Using STAINperfect immunostaining kit A, Dopamine (green) was detected within mouse embryonic mesencephalic neurons (E13.5) following the protocol optimized for whole mount samples. Stainings were performed using ImmuSmol rabbit anti-Dopamine polyclonal Ab (IS1005) and standard mouse anti-TH antibody. Fluorescent conjugated antibodies were used and images acquired by confocal imaging.
DatasheetMSDS
The anti-dopamine antibody IS1005 is a rabbit polyclonal antibody developped to ensure superior affinity and specificity. Combined with the STAINperfect immunostaining kit A, the antibody allows direct dopamine visualization in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| References | 5 citations |
| Format | 50µl (approx. 40 tissue sections) |
Product overview
| Product name | Dopamine antibody – Rabbit pAb |
| Synonyms | Anti-Dopamine antibody Anti-3,4-dihydroxyphenethylamine antibody Anti-DA antibody Anti-hydroxytyramine antibody Anti-oxytyramine |
| Immunogen | Conjugated Dopamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Dopamine antibody did not show any significant cross reactivity with Tyramine and L-Dopa conjugates |
| Volume | 50µl |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for dopamine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citation
- Zhang et al. Apoptosis signal regulating kinase 1 deletion mitigates α-synuclein pre-formed fibril propagation in mice. 2020 January.
- Smits et al. Modeling Parkinson’s disease in midbrain-like organoids. Nature, Parkinson’s Disease. 2019 April 05.
- Smits et al. Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids. bioRxiv. 2019 March 28.
- DeGregorio et al. miR-34b/c Regulates Wnt1 and Enhances Mesencephalic Dopaminergic Neuron Differentiation. Stem Cell Reports. 2018 Apr 10.
- Monzel et al. Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells. Stem Cell Reports. 2017 May 9
