Antibodies & immunoassays for small molecules Fast Histamine ELISA kit – HistaSure™ – Any fish sample
Ref: FC-E-3600
HistamineFood safetyResearch ELISA kits (RUO)ELISA
+Fast ELISA kit for histamine monitoring in fish samples
DatasheetProtocolMSDS
This fast Histamine ELISA kit was validated and certified by the AOAC Research Institute as an effective method for the quantitative measurement of histamine in fresh, frozen, canned fish samples and fish meals. Performed within 30 minutes, the assay can detect histamine levels between 0,44ppm and 300ppm in any fish sample, thus complying with regulatory requirements.
(Only available for customers outside of France)
| Sample type | 10g fresh fish, frozen fish, canned fish or fish meal |
| Samples/kit | 40 |
| Sensitivity | 0.44ppm |
| Range | 0 / 3.0 – 300ppm |
| Assay time | 25 min |
| Reactivity | Reacts with all species |
Product overview
| Product name | Fast Histamine ELISA kit – Fish samples – HistaSure™ |
| Description | Fast-track Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in fresh fish, frozen fish, canned fish or fish meals |
| Labels | AOAC Performance Tested Method #021402 |
| Format | 48-well plate |
| Samples | 10g fish sample |
| Reactivity | Reacts with all species |
| Standard range | 0 / 3.0 – 300ppm |
| Sensitivity |
0.44ppm |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as L-Tryptophan, Tryptamine, 3-Methyl-Histamine, L-Histidine, L-Tyrosine, L-Phenylalanine, Tyramine, Cadaverine, Spermine, Putrescine, Trimethylamine |
| Assay time | Sample acylation 5 min, ELISA 20 min |
| Storage | Store at 2-8°C for up to 12 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | Fresh/Frozen fish: keep fish frozen until analysis. Thaw samples under refrigeration or in cold water (no heated water bath). Store samples refrigerated (2-8°C) prior to testing. Blend samples until homogeneous.
Canned fish: Blend can content until homogeneous. Canned marine products packed in oil, sauce, brine or broth: Drain for 2 minutes and blend until homogeneous. Fish meal: Blend sample until homogeneous. |
| Extraction | Weigh 10g of sample, add 240ml ultra-pure water and homogenize for 1-2 minutes with blender. Filter/centrifuge homogeneate. Use 50µl of extract for acylation. |
| Acylation | Pipette 50µl of control(s) and sample extracts in wells. Add acylation buffer. Incubate with acylation reagent for 5min at RT on a shaker. |
| ELISA | Histamine antiserum incubation (10 min), revelation and read steps (20 min). |
| Detailed protocol | Download instructions for use |
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